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KMID : 0380620150470010027
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Korean Journal of Food Science and Technology
2015 Volume.47 No. 1 p.27 ~ p.36
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An Improved Analytical Method for the Determination of Qualitative and Quantitative Characteristics of Di- and Trisaccharides in Honey using GC and GC/MS
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Kim Jong-Bae
Jang Eun-Suk
Kim In-Suk
Lee Hee-Jin
Lee Hye-Jeong
Seo Hyun-Sun
Park Nam-Pyo
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Abstract
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The improved analytical method with gas chromatography (GC) and GC-mass spectrometry was established to identify and quantify disaccharides and trisacchrides in honey. In this method, the analysis of trimethylsilyl (TMS), TMSoxime and TMS-methoxime sugars takes into account the determination of a single peak of complete separation on the chromatogram. The number of possible peaks for the qualitative and quantitative determination of TMS, TMS-oxime, and TMS-methoxime sugars was 17, 22, and 25, respectively. This new analytical method allowed for the determination of diand trisaccharides in honey by TMS-oxime and TMS-methoxime derivatives. This study suggested that the improved method is more suitable and precise than the other analytical methods for the simultaneous determination of sugars in honey.
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KEYWORD
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honey, sugar, , trimethylsilyl (TMS), TMS-oxime, TMS-methoxime
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